BRAF antibody (Ser445)

Catalog No. ABIN544904

Product Details anti-BRAF Antibody

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Binding Specificity: Ser445

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of BRAF.

Cross-Reactivity: Human

Immunogen: A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S445 of human BRAF.

Application Details

Application Notes: Flow Cytometry (1:10-50)
Immunofluorescence (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-BRAF antibody (ABIN544904)

Hingorani, Jacobetz, Robertson, Herlyn, Tuveson: "Suppression of BRAF(V599E) in human melanoma abrogates transformation." in: Cancer research, Vol. 63, Issue 17, pp. 5198-202, (2003) (PubMed).

Lee, Yoo, Soung, Kim, Park, Kim, Lee, Park, Cho, Kim, Ko, Kim, Nam, Lee, Lee: "BRAF mutations in non-Hodgkin's lymphoma." in: British journal of cancer, Vol. 89, Issue 10, pp. 1958-60, (2003) (PubMed).

Davies, Bignell, Cox, Stephens, Edkins, Clegg, Teague, Woffendin, Garnett, Bottomley, Davis, Dicks, Ewing, Floyd, Gray, Hall, Hawes, Hughes, Kosmidou, Menzies, Mould, Parker, Stevens, Watt, Hooper et al.: "Mutations of the BRAF gene in human cancer. ..." in: Nature, Vol. 417, Issue 6892, pp. 949-54, (2002) (PubMed).

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: B-Raf proto-oncogene (BRAF Products)

Gene ID: 673

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy