PDPK1 antibody (N-Term)

Catalog No. ABIN545485

Product Details anti-PDPK1 Antibody

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of PDK1.

Cross-Reactivity: Human, Mouse

Immunogen: A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PDK1.

Application Details

Application Notes: ELISA (1:1000)
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-PDPK1 antibody (ABIN545485)

Sato, Fujita, Tsuruo: "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39360-7, (2002) (PubMed).

Frödin, Antal, Dümmler, Jensen, Deak, Gammeltoft, Biondi: "A phosphoserine/threonine-binding pocket in AGC kinases and PDK1 mediates activation by hydrophobic motif phosphorylation." in: The EMBO journal, Vol. 21, Issue 20, pp. 5396-407, (2002) (PubMed).

King, Zenke, Dawson, Dutil, Newton, Hemmings, Bokoch: "Sphingosine is a novel activator of 3-phosphoinositide-dependent kinase 1." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18108-13, (2000) (PubMed).

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1 (PDPK1 Products)

Synonyms: pdpk1 antibody, MGC82080 antibody, PDK1 antibody, PDPK2 antibody, PRO0461 antibody, Pdk1 antibody, zgc:153787 antibody, 3'-phosphoinositide-dependent protein kinase 1 antibody, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 antibody, ATPDK1 antibody, AtPDK1 antibody, T32M21.110 antibody, T32M21_110 antibody, zgc:77318 antibody, 3-phosphoinositide dependent protein kinase 1 antibody, 3-phosphoinositide dependent protein kinase 1 L homeolog antibody, 3-phosphoinositide dependent protein kinase-1 antibody, 3-phosphoinositide dependent protein kinase 1a antibody, 3-phosphoinositide-dependent protein kinase 1 antibody, 3'-phosphoinositide-dependent protein kinase 1 antibody, 3-phosphoinositide dependent protein kinase 1b antibody, PDPK1 antibody, pdpk1.L antibody, pdpk1 antibody, Pdpk1 antibody, pdpk1a antibody, LOC100380750 antibody, pdk-1 antibody, PDK1 antibody, pdpk1b antibody

Gene ID: 5163

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals