ELISA (1:1000) Western Blot (1:50-200) Immunohistochemistry (1:50-100) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Clark, Gurney, Abaya, Baker, Baldwin, Brush, Chen, Chow, Chui, Crowley, Currell, Deuel, Dowd, Eaton, Foster, Grimaldi, Gu, Hass, Heldens, Huang, Kim, Klimowski, Jin, Johnson, Lee, Lewis, Liao, Mark et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. ..." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, (2003) (PubMed).
Vucic, Deshayes, Ackerly, Pisabarro, Kadkhodayan, Fairbrother, Dixit: "SMAC negatively regulates the anti-apoptotic activity of melanoma inhibitor of apoptosis (ML-IAP)." in: The Journal of biological chemistry, Vol. 277, Issue 14, pp. 12275-9, (2002) (PubMed).
Gazzaniga, Gradilone, Giuliani, Gandini, Silvestri, Nofroni, Saccani, Frati, Aglianò: "Expression and prognostic significance of LIVIN, SURVIVIN and other apoptosis-related genes in the progression of superficial bladder cancer." in: Annals of oncology : official journal of the European Society for Medical Oncology / ESMO, Vol. 14, Issue 1, pp. 85-90, (2002) (PubMed).