Immunohistochemistry (1:50-1:100) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS, 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Connor, Kotchetkov, Cariou, Resch, Lupetti, Beniston, Melchior, Hengst, Slingerland: "CRM1/Ran-mediated nuclear export of p27(Kip1) involves a nuclear export signal and links p27 export and proteolysis." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 201-13, (2003) (PubMed).
Chopra, Fernandez De Mattos, Lam, Mann: "Jab1 co-activation of c-Jun is abrogated by the serine 10-phosphorylated form of p27Kip1." in: The Journal of biological chemistry, Vol. 277, Issue 36, pp. 32413-6, (2002) (PubMed).