BRAF antibody (pSer446)

Catalog No. ABIN548454

Product Details anti-BRAF Antibody

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Binding Specificity: pSer446

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of BRAF .

Specificity: This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).

Cross-Reactivity: Human, Mouse, Rat

Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S446 of human BRAF. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).

Application Details

Application Notes: ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (50 % glycerol, 1 mg/mL BSA, 0.05 % sodium azide).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-BRAF antibody (ABIN548454)

Brummer, Martin, Herzog, Misawa, Daly, Reth: "Functional analysis of the regulatory requirements of B-Raf and the B-Raf(V600E) oncoprotein." in: Oncogene, Vol. 25, Issue 47, pp. 6262-76, (2006) (PubMed).

Karbowniczek, Robertson, Henske: "Rheb inhibits C-raf activity and B-raf/C-raf heterodimerization." in: The Journal of biological chemistry, Vol. 281, Issue 35, pp. 25447-56, (2006) (PubMed).

Wilhelm, Carter, Tang, Wilkie, McNabola, Rong, Chen, Zhang, Vincent, McHugh, Cao, Shujath, Gawlak, Eveleigh, Rowley, Liu, Adnane, Lynch, Auclair, Taylor, Gedrich, Voznesensky, Riedl, Post, Bollag et al.: "BAY 43-9006 exhibits broad spectrum oral antitumor activity and targets the RAF/MEK/ERK pathway and receptor tyrosine kinases involved in tumor progression and angiogenesis. ..." in: Cancer research, Vol. 64, Issue 19, pp. 7099-109, (2004) (PubMed).

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: B-Raf proto-oncogene (BRAF Products)

Synonyms: B-RAF1 antibody, BRAF1 antibody, NS7 antibody, RAFB1 antibody, C-RMIL antibody, RMIL antibody, B-Raf antibody, braf1 antibody, rafb1 antibody, b-raf1 antibody, sierrabraf antibody, BRAF antibody, 9930012E13Rik antibody, AA120551 antibody, AA387315 antibody, AA473386 antibody, B-raf antibody, Braf-2 antibody, Braf2 antibody, C230098H17 antibody, C87398 antibody, D6Ertd631e antibody, braf antibody, fc48g01 antibody, wu:fc48g01 antibody, B-Raf proto-oncogene, serine/threonine kinase antibody, B-Raf proto-oncogene, serine/threonine kinase S homeolog antibody, Braf transforming gene antibody, si:dkey-222f8.3 antibody, BRAF antibody, Braf antibody, braf.S antibody, braf antibody, si:dkey-222f8.3 antibody

Gene ID: 673

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy