PKC alpha antibody (pSer657, pTyr658)

Catalog No. ABIN548457

Product Details anti-PKC alpha Antibody

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Binding Specificity: pSer657, pTyr658

Reactivity: Human, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKCA.

Specificity: This sequence is similar to a dual phosphorylation site found in PKCbI (Ser-661/Tyr-662). These human sequences are highly conserved in rat and mouse PKCa and PKCbI. In addition,

Cross-Reactivity: Human, Rabbit, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S657/Y658 of human PRKCA.

Application Details

Application Notes: ELISA (1:2000)
Immunoprecipitation (5-10 µL)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (50 % glycerol, 1 mg/mL BSA, 0.05 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-PKC alpha antibody (ABIN548457)

Kawakami, Kitaura, Yao, McHenry, Kawakami, Newton, Kang, Kato, Leitges, Rawlings, Kawakami: "A Ras activation pathway dependent on Syk phosphorylation of protein kinase C." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 16, pp. 9470-5, (2003) (PubMed).

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PRKCA (PKCa Products)

Synonyms: pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody

Gene ID: 5578

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling