RAGE antibody (Middle Region)

Catalog No. ABIN548553

Product Details anti-RAGE Antibody

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAGE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of AGER.

Specificity: The synthetic peptide of this antibody different from the mouse and rat sequence by two amino acids.

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Immunogen: A synthetic peptide corresponding to amino acids at middle region of human AGER.

Isotype: IgG

Application Details

Application Notes: Western Blot (1 μg/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1 μg/mL)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.9 mg NaCl, 0.2 mg Na₂HPO₄ (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.

References for anti-RAGE antibody (ABIN548553)

Bierhaus, Haslbeck, Humpert, Liliensiek, Dehmer, Morcos, Sayed, Andrassy, Schiekofer, Schneider, Schulz, Heuss, Neundörfer, Dierl, Huber, Tritschler, Schmidt, Schwaninger, Haering, Schleicher, Kasper et al.: "Loss of pain perception in diabetes is dependent on a receptor of the immunoglobulin superfamily. ..." in: The Journal of clinical investigation, Vol. 114, Issue 12, pp. 1741-51, (2004) (PubMed).

Chavakis, Bierhaus, Al-Fakhri, Schneider, Witte, Linn, Nagashima, Morser, Arnold, Preissner, Nawroth: "The pattern recognition receptor (RAGE) is a counterreceptor for leukocyte integrins: a novel pathway for inflammatory cell recruitment." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1507-15, (2003) (PubMed).

Taguchi, Blood, del Toro, Canet, Lee, Qu, Tanji, Lu, Lalla, Fu, Hofmann, Kislinger, Ingram, Lu, Tanaka, Hori, Ogawa, Stern, Schmidt: "Blockade of RAGE-amphoterin signalling suppresses tumour growth and metastases." in: Nature, Vol. 405, Issue 6784, pp. 354-60, (2000) (PubMed).

Target Details for RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: RAGE / AGER (AGER Products)

Synonyms: RAGE antibody, AGER antibody, advanced glycosylation end-product specific receptor antibody, advanced glycosylation end product-specific receptor antibody, MAPK/MAK/MRK overlapping kinase antibody, AGER antibody, Ager antibody, LOC719012 antibody

Gene ID: 177

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins