Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr75) antibody

Details for Product No. ABIN550194
Target
Binding Specificity
pThr75
30
19
15
9
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow
124
109
95
13
11
11
8
5
3
3
3
1
1
1
1
Host
Rabbit
121
7
1
1
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
5
5
5
5
5
3
2
2
2
2
2
2
1
Application
Western Blotting (WB)
88
44
41
27
23
16
7
6
5
4
4
1
1
Options
Purpose Rabbit polyclonal antibody raised against synthetic phosphopeptide of Ppp1r1b.
Immunogen Synthetic phosphopeptide corresponding to residues surrounding T75 of rat Ppp1r1b.
Cross-Reactivity Dog (Canine), Chicken, Human, Mouse (Murine), Primate, Rat (Rattus)
Target
Alternative Name DARPP32 (PPP1R1B Antibody Abstract)
Application Notes Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50 % glycerol, 10 % BSA)
Storage -20 °C
Storage Comment Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Images
Image no. 1 for anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr75) antibody (ABIN550194) Western blot of rat caudate lysate showing specific immunolabeling of the ~32k Ppp1r1...
Product cited in: Lindskog, Svenningsson, Pozzi, Kim, Fienberg, Bibb, Fredholm, Nairn, Greengard, Fisone: "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine." in: Nature, Vol. 418, Issue 6899, pp. 774-8, 2002 (PubMed).

Svenningsson, Tzavara, Witkin, Fienberg, Nomikos, Greengard: "Involvement of striatal and extrastriatal DARPP-32 in biochemical and behavioral effects of fluoxetine (Prozac)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 3182-7, 2002 (PubMed).

Maldve, Zhang, Ferrani-Kile, Schreiber, Lippmann, Snyder, Fienberg, Leslie, Gonzales, Morrisett: "DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens." in: Nature neuroscience, Vol. 5, Issue 7, pp. 641-8, 2002 (PubMed).