ASK1 antibody (pSer83)

Catalog No. ABIN550447

Product Details anti-ASK1 Antibody

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Binding Specificity: pSer83

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit polyclonal antibody raised against synthetic phosphpeptide of MAP3K5.

Specificity: This phospho specific polyclonal antibody reacts human pS83 ASK1.

Cross-Reactivity: Human, Mouse

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S83 of human MAP3K5.

Application Details

Application Notes: ELISA (1:5000-1:10000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-ASK1 antibody (ABIN550447)

Zou, Tsutsui, Ray, Blomquist, Ichijo, Ucker, Kiyokawa: "The cell cycle-regulatory CDC25A phosphatase inhibits apoptosis signal-regulating kinase 1." in: Molecular and cellular biology, Vol. 21, Issue 14, pp. 4818-28, (2001) (PubMed).

Matsuzawa, Ichijo: "Molecular mechanisms of the decision between life and death: regulation of apoptosis by apoptosis signal-regulating kinase 1." in: Journal of biochemistry, Vol. 130, Issue 1, pp. 1-8, (2001) (PubMed).

Morita, Saitoh, Tobiume, Matsuura, Enomoto, Nishitoh, Ichijo: "Negative feedback regulation of ASK1 by protein phosphatase 5 (PP5) in response to oxidative stress." in: The EMBO journal, Vol. 20, Issue 21, pp. 6028-36, (2001) (PubMed).

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: MEKK5 / ASK1 (MAP3K5 Products)

Synonyms: ASK1 antibody, MAPKKK5 antibody, MEKK5 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MUD21.11 antibody, MUD21_11 antibody, 7420452D20Rik antibody, ASK antibody, Mekk5 antibody, Ask1 antibody, RGD1306565 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MAP3K5 antibody, MAPKKK5 antibody, Map3k5 antibody, map3k5 antibody

Gene ID: 4217

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response