Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Purpose
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC27.
Specificity
Reactivity occurs against human CDC27 pT244 protein and This antibody is specific to the phosphorylated form of the protein. This antibody does not cross-react with CDC27 phosphorylated at other sites.
Cross-Reactivity
Chicken, Chimpanzee, Cow, Dog, Human, Mouse, Rat
Characteristics
Antibody Reactive Against Synthetic Peptide.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T244 of human CDC27.
ELISA (1:8000-1:30000) Western Blot (1:300-1:2000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Gabellini, Colaluca, Vodermaier, Biamonti, Giacca, Falaschi, Riva, Peverali: "Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression." in: The EMBO journal, Vol. 22, Issue 14, pp. 3715-24, (2003) (PubMed).
Topper, Campbell, Tugendreich, Daum, Burke, Hieter, Gorbsky: "The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis." in: Cell cycle (Georgetown, Tex.), Vol. 1, Issue 4, pp. 282-92, (2002) (PubMed).
Target
CDC27
(Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))