ATG14 antibody (Middle Region)

Catalog No. ABIN5516479

Product Details anti-ATG14 Antibody

Target: ATG14 (ATG14 Autophagy Related 14 (ATG14))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG14 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: NEEMEKNSEG LLKTKEKNQK LYSRAQRHQE KKEKIQRHNR KLGDLVEKKT

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATG14

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATG14

Target: ATG14 (ATG14 Autophagy Related 14 (ATG14))

Alternative Name: ATG14 (ATG14 Products)

Synonyms: ATG14L antibody, BARKOR antibody, KIAA0831 antibody, 4832427M01 antibody, Atg14L antibody, D14Ertd114e antibody, D14Ertd436e antibody, RGD1304610 antibody, autophagy related 14 antibody, ATG14 antibody, Atg14 antibody

Background: Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism.

Alias Symbols: ATG14L, BARKOR, KIAA0831

Protein Size: 492

Gene ID: 22863

NCBI Accession: NM_014924, NP_055739

UniProt: Q6ZNE5

Pathways: Autophagy