FLT1 antibody (N-Term)

Catalog No. ABIN5518696

Product Details anti-FLT1 Antibody

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Binding Specificity: AA 279-299, N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Vascular endothelial growth factor receptor 1(FLT1) detection. Tested with WB in Mouse.

Sequence: IRQRIDRSHS HNNVFHSVLK I

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Vascular endothelial growth factor receptor 1(FLT1) detection. Tested with WB in Mouse.
Gene Name: fms-related tyrosine kinase 1
Protein Name: Vascular endothelial growth factor receptor 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse FLT1(279-299aa IRQRIDRSHSHNNVFHSVLKI).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, The detection limit for FLT1 is approximately 1.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-FLT1 antibody (ABIN5518696)

Liu, Kuang, Wu, Jin, Sun: "A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo." in: Scientific reports, Vol. 6, pp. 26722, (2018) (PubMed).

Wu, You, Ma, Li, Yuan, Li, Ye, Liu, Yao, Chen, Lai, Yang: "Role of transient receptor potential ion channels and evoked levels of neuropeptides in a formaldehyde-induced model of asthma in BALB/c mice." in: PLoS ONE, Vol. 8, Issue 5, pp. e62827, (2013) (PubMed).

Target Details for FLT1

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Alternative Name: FLT1 (FLT1 Products)

Background: Vascular endothelial growth factor receptor 1(FLT1) is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family. It is mapped to 13q12. The deduced 1,338-amino acid protein has a calculated molecular mass of 150.6 kD. It has a 758-amino acid extracellular domain, followed by a 22-amino acid transmembrane region and a 558-amino acid cytoplasmic region containing a cluster of basic amino acids and a tyrosine kinase domain. sFLT-1 was identified in placenta, adult lung, kidney, liver and uterus. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation.

Synonyms: EC 2.7.10.1 antibody|Flt 1 antibody|FLT antibody|Flt-1 antibody|Flt1 antibody|Fms like tyrosine kinase 1 antibody|Fms related tyrosine kinase 1(vascular endothelial growth factor/vascular permeability factor receptor) antibody|Fms related tyrosine kinase 1 antibody|Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody|Fms-like tyrosine kinase 1 antibody|FRT antibody|Soluble VEGF receptor 1 14 antibody|Soluble VEGFR1 variant 2 antibody|Soluble VEGFR1 variant 21 antibody|Tyrosine protein kinase FRT antibody|Tyrosine protein kinase receptor FLT antibody|Tyrosine-protein kinase FRT antibody|Tyrosine-protein kinase receptor FLT antibody|Vascular endothelial growth factor receptor 1 antibody|Vascular endothelial growth factor receptor 1 Precursor antibody|Vascular endothelial growth factor vascular permeability factor receptor antibody|Vascular permeability factor receptor 1 antibody|Vascular permeability factor receptor antibody|VEGFR 1 antibody|VEGFR-1 antibody|VEGFR1 antibody|VGFR1_HUMAN antibody

UniProt: P35969

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals