ADIPOQ antibody (C-Term)

Catalog No. ABIN5518698

Product Details anti-ADIPOQ Antibody

Target: ADIPOQ (Adiponectin (ADIPOQ))

Binding Specificity: AA 224-244, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADIPOQ antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Adiponectin(ADIPOQ) detection. Tested with WB, IHC-P, IHC-F, ELISA in Human,Mouse,Rat.

Sequence: LYADNDNDST FTGFLLYHDT N

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Adiponectin(ADIPOQ) detection. Tested with WB, IHC-P, IHC-F, ELISA in Human,Mouse,Rat.
Gene Name: adiponectin, C1Q and collagen domain containing
Protein Name: Adiponectin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ADIPOQ(224-244aa LYADNDNDSTFTGFLLYHDTN), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for ADIPOQ is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-ADIPOQ antibody (ABIN5518698)

Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

Gao, Li, Zhang, Dai, Jiang, Liu, Zhang, Chen, Zhang: "Silencing of ADIPOQ efficiently suppresses preadipocyte differentiation in porcine." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 31, Issue 2-3, pp. 452-61, (2013) (PubMed).

Liu, Mu, Yuan, Wu, Liu, Zheng, Lian, Ren, Xu: "High salt intake fails to enhance plasma adiponectin in normotensive salt-sensitive subjects." in: Nutrition (Burbank, Los Angeles County, Calif.), Vol. 28, Issue 4, pp. 422-5, (2012) (PubMed).

Target Details for ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: ADIPOQ (ADIPOQ Products)

Synonyms: ADP antibody, DCAF9 antibody, 6720489L24Rik antibody, 9330209L04Rik antibody, Adp antibody, Mamdc1 antibody, RNSVSP4U antibody, SVSIV1 antibody, Svp4 antibody, ACDC antibody, ACRP30 antibody, ADIPQTL1 antibody, ADPN antibody, APM-1 antibody, APM1 antibody, GBP28 antibody, apM-1 antibody, 30kDa antibody, APN antibody, Acdc antibody, Acrp30 antibody, Ad antibody, adipo antibody, apM1 antibody, ADN antibody, acrpl antibody, adipo-b antibody, adipoql antibody, zgc:153584 antibody, adipoq antibody, WD and tetratricopeptide repeats 1 antibody, MAM domain containing glycosylphosphatidylinositol anchor 2 antibody, seminal vesicle secretory protein 4 antibody, adiponectin, C1Q and collagen domain containing antibody, adiponectin, C1Q and collagen domain containing, b antibody, adiponectin, C1Q and collagen domain containing L homeolog antibody, WDTC1 antibody, Mdga2 antibody, Svs4 antibody, ADIPOQ antibody, Adipoq antibody, adipoqb antibody, adipoq.L antibody

Background: ADIPOQ(Adipocyte-, C1q-, and Collagen Domain-Containing), also known as APM1, ADPN or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al.(2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al.(1999) showed that APM1 is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, they found links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Adiponectin is exclusively secreted from adipose tissue(and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.

Synonyms: 30 kDa adipocyte complement related protein antibody|30 kDa adipocyte complement-related protein antibody|ACDC antibody|ACRP 30 antibody|ACRP30 antibody|ADIPO_HUMAN antibody|Adipocyte antibody|Adipocyte C1q and collagen domain containing protein antibody|Adipocyte complement related 30 kDa protein antibody|Adipocyte complement related protein of 30 kDa antibody|Adipocyte complement-related 30 kDa protein antibody|Adiponectin antibody|AdipoQ antibody|Adipose most abundant gene transcript 1 antibody|Adipose most abundant gene transcript 1 protein antibody|Adipose specific collagen like factor antibody|ADIPQTL1 antibody|ADPN antibody|APM 1 antibody|apM-1 antibody|ApM1 antibody|C1q and collagen domain-containing protein antibody|GBP 28 antibody|GBP28 antibody|Gelatin binding protein antibody|Gelatin binding protein 28 antibody|Gelatin-binding protein antibody

UniProt: Q15848