ATP4b antibody (N-Term)

Catalog No. ABIN5518738

Product Details anti-ATP4b Antibody

Target: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Binding Specificity: AA 1-28, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP4b antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Potassium-transporting ATPase subunit beta(ATP4B) detection. Tested with WB in Human,Mouse,Rat.

Sequence: MAALQEKKTC GQRMEEFQRY CWNPDTGQ

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Potassium-transporting ATPase subunit beta(ATP4B) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: ATPase H+/K+ transporting beta subunit
Protein Name: Potassium-transporting ATPase subunit beta

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ATP4B (1-28aa MAALQEKKTCGQRMEEFQRYCWNPDTGQ), different from the related mouse and rat sequences by five amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ATP4b

Target: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Alternative Name: ATP4B (ATP4b Products)

Synonyms: AV080843 antibody, S76401S1 antibody, ATP6B antibody, ATPase, H+/K+ exchanging, beta polypeptide antibody, ATPase H+/K+ transporting beta subunit antibody, ATPase, H+/K+ exchanging, beta polypeptide S homeolog antibody, ATPase H+/K+ transporting alpha subunit antibody, Atp4b antibody, atp4b.S antibody, ATP4B antibody, ATP4A antibody

Background: This gene encodes the beta subunit of the gastric H+, K+-ATPase. The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion.

Synonyms: ATP4B | ATP6B | Parietal cell antigen | Proton pump beta chain | P51164

Gene ID: 496

UniProt: P51164

Pathways: Proton Transport