TNFRSF10B antibody (AA 233-440)

Catalog No. ABIN5518794

Product Details anti-TNFRSF10B Antibody

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Binding Specificity: AA 233-440

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF10B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 10B(TNFRSF10B) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 10B(TNFRSF10B) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: tumor necrosis factor receptor superfamily, member 10b
Protein Name: Tumor necrosis factor receptor superfamily member 10B

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DR5 recombinant protein (Position: K233-S440). Human DR5 shares 48.4% amino acid (aa) sequence identity with mouse DR5.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-TNFRSF10B antibody (ABIN5518794)

Liu, Zuo, Wang, Chen, Yang, Wang, Zhu: "miR-942 decreases TRAIL-induced apoptosis through ISG12a downregulation and is regulated by AKT." in: Oncotarget, Vol. 5, Issue 13, pp. 4959-71, (2014) (PubMed).

Target Details for TNFRSF10B

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Alternative Name: TNFRSF10B (TNFRSF10B Products)

Synonyms: TNFRSF10B antibody, dr5 antibody, cd262 antibody, killer antibody, trick2 antibody, trickb antibody, xDR-M1 antibody, xDR-M2 antibody, ztnfr9 antibody, trailr2 antibody, trick2a antibody, trick2b antibody, CD262 antibody, DR5 antibody, KILLER antibody, KILLER/DR5 antibody, TRAIL-R2 antibody, TRAILR2 antibody, TRICK2 antibody, TRICK2A antibody, TRICK2B antibody, TRICKB antibody, ZTNFR9 antibody, Ly98 antibody, MK antibody, tumor necrosis factor receptor superfamily, member 10b antibody, tumor necrosis factor receptor superfamily member 10b S homeolog antibody, tumor necrosis factor receptor superfamily member 10b L homeolog antibody, tumor necrosis factor receptor superfamily member 10b antibody, TNF receptor superfamily member 10b antibody, Tnfrsf10b antibody, TNFRSF10B antibody, tnfrsf10b.S antibody, tnfrsf10b.L antibody, tnfrsf10b antibody

Background: TNFRSF10B(Tumor necrosis factor receptor superfamily, member 10b) is a human gene. It is also known as DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5. The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. Mice have a homologous gene, tnfrsf10b that has been essential in the elucidation of the function of this gene in humans. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.By analysis of radiation hybrid panels, this gene is mapped to chromosome 8p22-p21. Northern blot analysis indicated that TRAILR2 was expressed as a 4.4-kb mRNA in all tissues tested, with the highest levels of expression in peripheral blood lymphocytes, spleen, and ovary.

Synonyms: CD262 antigen | Cytotoxic TRAIL receptor 2 | DR5 | Dr5 | Killer | KILLER | O14763 | TNFRSF10B | TR10B | trail r2 | trailr 2 | TRAILR2 | TRAIL-R2 | TRICK2 | TRICK2A | TRICK2B

Gene ID: 8795

UniProt: O14763

Pathways: p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity