Sca-1/Ly-6A/E antibody (Middle Region)

Catalog No. ABIN5518854

Product Details anti-Sca-1/Ly-6A/E Antibody

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Binding Specificity: AA 48-75, Middle Region

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sca-1/Ly-6A/E antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Lymphocyte antigen 6A-2/6E-1(Ly6a) detection. Tested with WB in Mouse.

Sequence: YPDGVCVTQE AAVIVDSQTR KVKNNLCL

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Lymphocyte antigen 6A-2/6E-1(Ly6a) detection. Tested with WB in Mouse.
Gene Name: lymphocyte antigen 6 complex, locus A
Protein Name: Lymphocyte antigen 6A-2/6E-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse Sca1/Ly6A/E (48-75aa YPDGVCVTQEAAVIVDSQTRKVKNNLCL).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Sca-1/Ly-6A/E antibody (ABIN5518854)

Xiang, Liang, Zhen-Yong, Liang-Jun, Zhong-Liang: "BMP9-induced osteogenetic differentiation and bone formation of muscle-derived stem cells." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 610952, (2012) (PubMed).

Target Details for Sca-1/Ly-6A/E

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Alternative Name: Ly6a (Ly6a Products)

Synonyms: Ly-6A.2 antibody, Ly-6A/E antibody, Ly-6E.1 antibody, Sca-1 antibody, Sca1 antibody, TAP antibody, lymphocyte antigen 6 complex, locus A antibody, Ly6a antibody

Background: Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs). It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in several non hematopoietic tissues and can be used to enrich progenitor cell populations other than HSC. It is suggested that Sca-1 could be involved in regulating both B and T cell activation.

Synonyms: Ly6a | Ly-6A.2/Ly-6E. | Ly-6A.2/Ly-6E.1 | Sca-1 | Sca1 | TAP

Gene ID: 110454

UniProt: P05533

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation