Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Immunogen
This CYP51A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human CYP51A1.
CYP51A1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Concentration
2 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
CYP51A1
(Cytochrome P450, Family 51, Subfamily A, Polypeptide 1 (CYP51A1))
CP51 antibody, CYP51 antibody, CYPL1 antibody, LDM antibody, P450-14DM antibody, P450L1 antibody, cyp51a1 antibody, cytochrome P450 family 51 subfamily A member 1 antibody, cytochrome P450, family 51, subfamily A, polypeptide 1 antibody, cytochrome P450 family 51 subfamily A member 1 L homeolog antibody, CYP51A1 antibody, cyp51a1 antibody, cyp51a1.L antibody
Background
CYP51A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol.