This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This RAPGEF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 529-555 amino acids from the C-terminal region of human RAPGEF5.
RAPGEF5
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
For WB starting dilution is: 1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
GFR (RAPGEF5)
(Rap Guanine Nucleotide Exchange Factor (GEF) 5 (RAPGEF5))
Members of the RAS (see HRAS, MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).