SCYL1 antibody (N-Term)

Catalog No. ABIN5537183

Product Details anti-SCYL1 Antibody

Target: SCYL1 (SCY1-Like 1 (SCYL1))

Binding Specificity: AA 156-185, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCYL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SCYL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the N-terminal region of human SCYL1.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SCYL1

Target: SCYL1 (SCY1-Like 1 (SCYL1))

Alternative Name: SCYL1 (SCYL1 Products)

Synonyms: DDBDRAFT_0189357 antibody, DDBDRAFT_0220502 antibody, DDB_0189357 antibody, DDB_0220502 antibody, GKLP antibody, NKTL antibody, NTKL antibody, P105 antibody, TAPK antibody, TEIF antibody, TRAP antibody, 2810011O19Rik antibody, C85140 antibody, Ntkl antibody, mdf antibody, mfd antibody, p105 antibody, N-terminal kinase-like protein antibody, SCY1 like pseudokinase 1 antibody, SCY1-like, kinase-like 1 L homeolog antibody, SCY1-like 1 (S. cerevisiae) antibody, scy1 antibody, PITG_12105 antibody, SCYL1 antibody, LOC592987 antibody, scyl1.L antibody, Scyl1 antibody

Background: SCYL1 forms multimers following transfection into COS-7 cells. SCYL1 forms a 300-kD trimer using crosslinking reagents. Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis. During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes. However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling. Endogenous variant 2 in HeLa cells showed a similar staining pattern. Centrosomal localization was independent of microtubules.

Molecular Weight: 90 kDa

Gene ID: 57410

UniProt: Q96KG9

Pathways: p53 Signaling