This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This Mouse Rnasel antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-167 amino acids from the N-terminal region of mouse Rnasel.
RNASEL
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
For WB starting dilution is: 1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
RNASEL
(Ribonuclease L (2',5'-Oligoisoadenylate Synthetase-Dependent) (RNASEL))
E230029I04Rik antibody, PRCA1 antibody, RNS4 antibody, ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) antibody, ribonuclease L antibody, Rnasel antibody, RNASEL antibody
Background
Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. Might play a central role in the regulation of mRNA turnover (By similarity).