USP8 antibody (C-Term)

Catalog No. ABIN5537693

Product Details anti-USP8 Antibody

Target: USP8 (Ubiquitin Specific Peptidase 8 (USP8))

Binding Specificity: AA 1058-1087, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen: This USP8 (UBPY) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1058-1087 amino acids from the C-terminal region of human USP8 (UBPY).

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for USP8

Target: USP8 (Ubiquitin Specific Peptidase 8 (USP8))

Alternative Name: USP8 (USP8 Products)

Synonyms: HumORF8 antibody, UBPY antibody, AI574262 antibody, AW557536 antibody, Ubpy antibody, mKIAA0055 antibody, ubiquitin carboxyl-terminal hydrolase 8 antibody, ubiquitin specific peptidase 8 antibody, LOC9310109 antibody, MGYG_00369 antibody, USP8 antibody, Usp8 antibody

Background: Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Molecular Weight: 128 kDa

Gene ID: 9101

UniProt: P40818