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IRS4 antibody (AA 633-647)

Verified IRS4 Reactivity: Mouse WB, ELISA, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN5539610
  • Target See all IRS4 Antibodies
    IRS4 (Insulin Receptor Substrate 4 (IRS4))
    Binding Specificity
    • 15
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 633-647
    Reactivity
    • 31
    • 22
    • 15
    Mouse
    Host
    • 36
    • 2
    Goat
    Clonality
    • 36
    • 2
    Polyclonal
    Conjugate
    • 10
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This IRS4 antibody is un-conjugated
    Application
    • 26
    • 26
    • 21
    • 10
    • 6
    • 6
    • 4
    • 3
    • 2
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Purpose
    Irs4 (mouse aa633-647)
    Sequence
    DRGTKERKEA KEVRD
    Cross-Reactivity
    Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Isotype
    IgG
    Top Product
    Discover our top product IRS4 Primary Antibody
  • Application Notes

    Western Blot: Approx 150 kDa band observed in Mouse Heart lysates (calculated MW of 130 kDa according to NP_034702.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 20

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunofluorescence: This product has been successfully used in IF on Mouse: Harriet M Jackson et al, BMC Genomics. 2013 Nov 25,14:831. PMID: 24274089.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Ikink, Boer, Bakker, Hilkens: "IRS4 induces mammary tumorigenesis and confers resistance to HER2-targeted therapy through constitutive PI3K/AKT-pathway hyperactivation." in: Nature communications, Vol. 7, pp. 13567, (2018) (PubMed).

  • Target
    IRS4 (Insulin Receptor Substrate 4 (IRS4))
    Alternative Name
    Irs4 (IRS4 Products)
    Synonyms
    IRS4 antibody, irs-4 antibody, py160 antibody, IRS-4 antibody, PY160 antibody, irs2 antibody, irs2-a antibody, insulin receptor substrate 4 antibody, insulin receptor substrate 4 S homeolog antibody, IRS4 antibody, irs4 antibody, Irs4 antibody, irs4.S antibody
    Background
    Irs4, insulin receptor substrate 4, IRS-4, RP23-415G1.3, phosphoprotein of 160 kDa, pp160
    Gene ID
    16370, 315350
    NCBI Accession
    NP_034702
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