Doublecortin antibody (Internal Region)

Catalog No. ABIN5539645

Product Details anti-Doublecortin Antibody

Target: Doublecortin (DCX)

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Doublecortin (mouse)

Sequence: PKASPTPQKT SAKS

Specificity: This antibody is expected to recognize all reported mouse isoforms (NP_001103692.1, NP_001103694.1, NP_034155.2). Reported variants represent identical protein: NP_001103692.1, NP_001103693.1

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6 kDa according to NP_001103692.1). Recommended concentration: 0.5-2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5μg/ml

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: Dcx (DCX Products)

Background: Dcx, doublecortin, Dbct, OTTMUSP00000020394, OTTMUSP00000020395, OTTMUSP00000020396, OTTMUSP00000020397, doublecortex, doublin, lis-X, lissencephalin-X, lissencephaly, X-linked (doublecortin), neuronal migration protein doublecortin

Gene ID: 1641, 13193, 84394

NCBI Accession: NP_001103692, NP_001103694, NP_034155