MMP 9 antibody (Internal Region)

Catalog No. ABIN5539846

Product Details anti-MMP 9 Antibody

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MMP 9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: MMP9

Sequence: KLDSVFEEPL SKK

Predicted Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx.80 kDa and 70 kDa bands observed in Human Peripheral Blood Mononucleocytes lysates. These bands correspond to the mature forms (http://www.uniprot.org/uniprot/P14780). This protein has a calculated MW of 78.5 kDa according to NP_004985.2, but the precursor is usually seen as 92 kDa or larger. Recommended concentration: 1-3 μg/mL. Approx.75 kDa band observed in Mouse Heart lysates (calculated MW of 80.5 kDa according to Mouse NP_038627.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MMP 9

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Alternative Name: MMP9 (MMP9 Products)

Synonyms: CLG4B antibody, GELB antibody, MANDP2 antibody, MMP-9 antibody, AW743869 antibody, B/MMP9 antibody, Clg4b antibody, pro-MMP-9 antibody, mmp-9 antibody, fgMMP-9 antibody, mmp9 antibody, ZFMMP-9 antibody, wu:fb02g06 antibody, wu:fb07b05 antibody, wu:fi98c09 antibody, wu:fj05a08 antibody, zgc:64165 antibody, clg4b antibody, gelb antibody, mandp2 antibody, matrix metallopeptidase 9 antibody, collagenase antibody, matrix metalloproteinase 9 antibody, matrix metallopeptidase 9 S homeolog antibody, MMP9 antibody, RB3913 antibody, BPSS0666 antibody, Sbal_3732 antibody, Bcer98_0486 antibody, Shew185_0630 antibody, Shal_3056 antibody, Sbal195_0657 antibody, Lbys_3550 antibody, Palpr_2084 antibody, Mmp9 antibody, mmp9 antibody, mmp9.S antibody

Background: MMP9, matrix metalloproteinase 9, gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase, HGNC:7176, CLG4B, GELB, 92kD type IV collagenase, gelatinaseB, macrophage gelatinase, matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase), type V collagenase

Gene ID: 4318

NCBI Accession: NP_004985

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events