Insulin antibody

Catalog No. ABIN5540065

Product Details anti-Insulin Antibody

Target: Insulin (INS)

Reactivity: Pig

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID)

Specificity: The reactivity of the antiserum is directed to Insulin as tested by ELISA and Immunoblotting. No reaction was obtained with other Swine serum proteins.

Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt Precipitation and purification of the IgG fraction by DEAE Chromatography

Immunogen: Insulin hormone is isolated from Porcine pancreas. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates in radioimmunoassay for the production of immunoconjugates with a selected marker to prepare immunoaffinity adsorbents by coupling to an artificial carrier in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent in Western blotting. This product is not pre-diluted. Recommended Working Dilutions : Histochemical and Cytochemical Use: are usually between 1/100-1/250. In ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2 Preservatives: None, as it may interfere with the antibody activity. No foreign proteins added

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: insulin (INS Products)

Synonyms: IDDM2 antibody, ILPR antibody, IRDN antibody, MODY10 antibody, ins1 antibody, xins antibody, ins1-a antibody, Insulin antibody, AA986540 antibody, Ins-2 antibody, InsII antibody, Mody antibody, Mody4 antibody, proinsulin antibody, zgc:109842 antibody, igf2-A antibody, ins antibody, ins-a antibody, ins-b antibody, insulin antibody, insulin precursor antibody, insulin II antibody, preproinsulin antibody, insulin L homeolog antibody, insulin S homeolog antibody, INS antibody, INS-IGF2 antibody, ins antibody, Ins antibody, PIN antibody, Ins2 antibody, ins.L antibody, ins.S antibody

Background: Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds.

UniProt: P01315

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation