CLIC5 antibody (Isoform 1)

Catalog No. ABIN5540213

Product Details anti-CLIC5 Antibody

Target: CLIC5 (Chloride Intracellular Channel 5 (CLIC5))

Binding Specificity: AA 160-173, Isoform 1

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLIC5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: This antibody recognizes CLIC5a, a 32 kDa protein which is component of actin complexes. Cross reactivity with CLIC5b was not determined.

Purification: Protein-A affinity chromatography (> 95% by SDS-PAGE)

Immunogen: KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a

Clone: CLIC5-02

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry. Western blot.

Protocol: 1. Wegner B, Al-Momany A, Kulak SC, Kozlowski K, Obeidat M, Jahroudi N, Paes J, Berryman M, Ballermann BJ: CLIC5A, a component of the ezrin-podocalyxin complex in glomeruli, is a determinant of podocyte integrity. Am J Physiol Renal Physiol. 2010 Jun298(6):F1492-503.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 95 % by SDS-PAGE) Buffer System: Phosphate buffered saline (PBS) Preservatives: 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CLIC5

Target: CLIC5 (Chloride Intracellular Channel 5 (CLIC5))

Alternative Name: clic5 (CLIC5 Products)

Synonyms: MST130 antibody, MSTP130 antibody, 5730531E12Rik antibody, B330005L24 antibody, Gm322 antibody, jbg antibody, clic5 antibody, im:6907594 antibody, zgc:77538 antibody, CLIC5 antibody, zgc:101827 antibody, chloride intracellular channel 5 antibody, chloride intracellular channel 5b antibody, chloride intracellular channel 5a antibody, chloride intracellular channel 5 L homeolog antibody, CLIC5 antibody, Clic5 antibody, clic5b antibody, clic5a antibody, clic5.L antibody

Background: CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.

UniProt: Q9NZA1

Pathways: Sensory Perception of Sound