EDNRB
Reactivity: Human
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot. Immunocytochemistry. Immunohistochemistry on Paraffin Embedded Sections : 4-11 μg/mL Antigen Retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS containing 0.09 % Sodium Azide as preservative
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-80 °C
Storage Comment
Store undiluted at 2-8°C for one month or (in aliquots) at -20 to -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Tanaka, Sho, Takayama, Wakatsuki, Matsumoto, Migita, Ito, Hamada, Nakajima: "Endothelin B receptor expression correlates with tumour angiogenesis and prognosis in oesophageal squamous cell carcinoma." in: British journal of cancer, Vol. 110, Issue 4, pp. 1027-33, (2014) (PubMed).
Jones, Brockbank, Mobbs, Le Good, Soma-Haddrick, Heuze, Langham, Timms, Newham, Needham: "The orphan G-protein coupled receptor RDC1: evidence for a role in chondrocyte hypertrophy and articular cartilage matrix turnover." in: Osteoarthritis and cartilage, Vol. 14, Issue 6, pp. 597-608, (2006) (PubMed).
The Endothelin Receptor ETB is expressed in most tissues. Mutations of the receptor gene are responsible for Hirschsprung disease type 2, a multigenic disorder with phenotypes such as bicolored irides, megacolon, hypopigmentation, and hearing loss. The ETB receptor has similar affinity for all three endothelins and activates a phosphatidylinositol-calcium second messenger system. A splice variant, termed SVR, has been described the sequence of the ETB-SVR receptor is identical to ET-B receptor, with the exception of the intracellular C-terminal domain. While both splice variants bind endothelin ET1, they exhibit different responses, which suggests that they may be functionally distinct. The endothelin B receptor has been reported to be expressed in adrenal, aorta, artery, brain, breast, eye, epididymis, ganglion, heart, kidney, liver, lung, ovary, placenta, prostate, skin, testis, uterus, and vessel.