VEGFR2/CD309 antibody (3rd Extracellular Domain)

Catalog No. ABIN5541982

Product Details anti-VEGFR2/CD309 Antibody

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Binding Specificity: 3rd Extracellular Domain

Reactivity: Human, Pig

Host: Rat

Clonality: Monoclonal

Conjugate: This VEGFR2/CD309 antibody is un-conjugated

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Specificity: This antibody detects VEGFR2 (epitope on the third extracellular immunoglobulin-like domain).

Purification: Protein G Chromatography

Immunogen: genetic immunisation with cDNA encoding Human VEGFR2. Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.

Clone: BEP-5D8

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. Cell based ELISA:

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate buffered saline, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Alternative Name: cd309,vegfr-2,flk-1 (VEGFR2 Products)

Synonyms: CD309 antibody, FLK1 antibody, VEGFR antibody, VEGFR2 antibody, 6130401C07 antibody, Flk-1 antibody, Flk1 antibody, Krd-1 antibody, Ly73 antibody, VEGFR-2 antibody, sVEGFR-2 antibody, Vegfr-2 antibody, FLK-1 antibody, flk-1 antibody, vegfr-2 antibody, KDR antibody, FLK/KDR antibody, flk1 antibody, flk1b antibody, kdrb antibody, si:busm1-205d10.1 antibody, si:ch211-254j6.1 antibody, si:ch211-278f21.4 antibody, wu:fc31a09 antibody, fk52c05 antibody, flk antibody, kdr antibody, kdra antibody, vegfr2 antibody, vegfr4 antibody, vegr2 antibody, wu:fk52c05 antibody, kinase insert domain receptor antibody, kinase insert domain protein receptor antibody, vascular endothelial growth factor receptor kdr-like antibody, kinase insert domain receptor (a type III receptor tyrosine kinase) antibody, kinase insert domain receptor like antibody, KDR antibody, Kdr antibody, LOC100136647 antibody, kdr antibody, kdrl antibody

Background: The vascular endothelial growth factor receptor-2 (VEGFR-2) plays an important role in the control of angiogenesis. This molecule is expressed on the surface of circulating endothelial stem cells as well as on adult vascular endothelial cells and other cell types (1). It is a 200- kDa type I transmembrane protein, consisting of a 19 amino acid leader peptide, a 744 amino acid extracellular domain made up of 7 immunoglobulin-like domains, a 24 amino acid transmembrane region and a 568 amino acid cytoplasmic domain. Interactions with VEGF ligands will stimulate angiogenesis, required in wound healing but also misused by tumours to promote their vascularization (2). The current monoclonal antibody was developed by genetic immunization against the complete extracellular domain of human VEGFR-2 and has been shown to be specific for the native target molecule and its counterpart in pigs using FACscan analyses on viable, transiently-transfected human BOSC23 cells (see Fig. 1). It binds to an epitope on the third extracellular immunoglobulin-like domain.

UniProt: P35968

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling