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NeuN antibody (AA 1-140)

RBFOX3 Reactivity: Human, Mouse WB, IHC, ELISA, ICC, FACS Host: Mouse Monoclonal 1H3B2 unconjugated
Catalog No. ABIN5542704
  • Target See all NeuN (RBFOX3) Antibodies
    NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
    Binding Specificity
    • 16
    • 5
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    AA 1-140
    Reactivity
    • 52
    • 43
    • 38
    • 16
    • 4
    • 3
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 42
    • 14
    • 2
    Mouse
    Clonality
    • 32
    • 26
    Monoclonal
    Conjugate
    • 27
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This NeuN antibody is un-conjugated
    Application
    • 36
    • 18
    • 18
    • 14
    • 14
    • 13
    • 11
    • 10
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Immunogen
    Purified recombinant fragment of human RBFOX3 (AA 1-140) expressed in E. Coli.
    Clone
    1H3B2
    Isotype
    IgG2a
  • Application Notes
    ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: , ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
    Restrictions
    For Research Use only
  • Validation #104496 (Western Blotting)
    'Independent Validation' Badge
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #104496
    Date
    08/02/2023
    Antigen
    RBFOX3
    Lot Number
    160425
    Method validated
    Western Blotting
    Positive Control

    Adult mouse brain and cerebellum

    Negative Control
    Notes

    The NeuN antibody ABIN5542704 works in WB at a 1:1000 dilution with sensitive ECL substrate on adult mouse brain and cerebellum extracts. Non-specific bands are also detected.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN5542704
    Secondary Antibody

    HRP-conjugated goat anti-mouse IgG (Sigma-Aldrich, NA931VS)

    Full Protocol
    • Homogenize tissues with cold lysis buffer containing 50 mM Tris HCl, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, and 1% protease inhibitor (Sigma P8340) using an ultrasonic homogenizer (MSE, SoniPrep 150) with 16 amplitude, 20 s on, 10 s off pulse for 60 s.
    • Centrifuge tissue homogenates at 13,000 rpm for 20 min at 4 °C.
    • Collect supernatants and determine total protein content using a Bradford assay.
    • Denature 50 µg of total protein for 5 min at 90 °C and subsequently separate them on a denaturing 12% PAGE-SDS gel alongside a Precision Plus Protein Dual Color Standard (Bio-Rad, 160374).
    • Electro-transfer proteins onto nitrocellulose membrane (Amersham Biosciences, RPN203D) ON in the cold room.
    • Wash membrane 3x for 10 min with 0.01 M PBS containing 0.1% Tween-20 (PBST).
    • Block membrane with PBST containing 2% bovine serum albumin for 1 h at RT.
    • Incubate membrane with primary mouse anti-NeuN antibody (antibodies-online, ABIN5542704, lot 160425) diluted 1:1,000 in PBST ON at 4 °C.
    • Wash membrane 3x 10 min with PBST.
    • Incubate membrane with secondary HRP-conjugated goat anti-mouse IgG (Sigma-Aldrich, NA931VS) diluted 1:1,000 in PBST for 1 h at RT.
    • Wash membrane 3x 10 min with PBST.
    • Visualize proteins with WesternBright Sirius HRP substrate (Advansta) using a ChemiDoc Imaging System.
    Experimental Notes

    Non-specific bands were also detected in adult mouse cerebellum tissue extracts, but not on laser-microdissected Purkinje neurons.

  • Format
    Liquid
    Buffer
    PBS with 0.05 % sodium azide
    Storage
    4 °C,-20 °C
    Storage Comment
    4°C, -20°C for long term storage
  • Target
    NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
    Alternative Name
    RBFOX3 (RBFOX3 Products)
    Synonyms
    FOX-3 antibody, FOX3 antibody, HRNBP3 antibody, NEUN antibody, Fox-3 antibody, Hrnbp3 antibody, NeuN antibody, Neuna60 antibody, Neun antibody, RGD1560070 antibody, RNA binding fox-1 homolog 3 antibody, RNA binding protein, fox-1 homolog (C. elegans) 3 antibody, RNA binding protein, fox-1 homolog 3 antibody, RBFOX3 antibody, Rbfox3 antibody
    Background
    RBFOX3 (RNA Binding Protein, Fox-1 Homolog (C. Elegans) 3) is a Protein Coding gene. Diseases associated with RBFOX3 include vertebral artery occlusion and tuberculum sellae meningioma. Among its related pathways are Neuroscience. GO annotations related to this gene include nucleic acid binding and nucleotide binding. An important paralog of this gene is RBFOX1.,
    Molecular Weight
    28.3kDa
    HGNC
    23492
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