Tyrosine Hydroxylase antibody

Catalog No. ABIN372734

Product Details anti-Tyrosine Hydroxylase Antibody

Target: Tyrosine Hydroxylase (TH)

Reactivity: Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Specificity: Specific for the ~60k tyrosine hydroxylase protein. The antibody recognizes all mammalian and at least some non-mammalian forms of the enzyme in Western blots and in Immunohistochemistry/Immunofluorescence.

Cross-Reactivity (Details): Species reactivity (tested):Mammalia.

Purification: Affinity Chromatography on Protein A.

Immunogen: SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1/1,000. Immunofluorescence 1/1000 (Frozen Sections). Immunohistochemistry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C or (in aliquots) at -80 °C for long term storage.

References for anti-Tyrosine Hydroxylase antibody (ABIN372734)

Grapensparr, Vasylovska, Li, Olerud, Jansson, Kozlova, Carlsson: "Co-transplantation of human pancreatic islets with post-migratory neural crest stem cells increases β-cell proliferation and vascular and neural regrowth." in: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 4, pp. E583-90, (2015) (PubMed).

Kirane, Toombs, Larsen, Ostapoff, Meshaw, Zaknoen, Brekken, Burrows: "Epithelial-mesenchymal transition increases tumor sensitivity to COX-2 inhibition by apricoxib." in: Carcinogenesis, Vol. 33, Issue 9, pp. 1639-46, (2012) (PubMed).

Kish, Kalasinsky, Derkach, Schmunk, Guttman, Ang, Adams, Furukawa, Haycock: "Striatal dopaminergic and serotonergic markers in human heroin users." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 24, Issue 5, pp. 561-7, (2001) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).

Witkovsky, Gabriel, Haycock, Meller: "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina." in: Journal of chemical neuroanatomy, Vol. 19, Issue 2, pp. 105-16, (2000) (PubMed).

Zhu, Klimek, Haycock, Ordway: "Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain." in: Journal of neuroscience methods, Vol. 99, Issue 1-2, pp. 37-44, (2000) (PubMed).

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine 3-Monooxygenase (TH) (TH Products)

Synonyms: DYT14 antibody, DYT5b antibody, TYH antibody, The antibody, CG10118 antibody, DH65B antibody, DTH antibody, Dmel\\CG10118 antibody, Pale antibody, Ple antibody, TH antibody, Th antibody, VII antibody, dTH1 antibody, dTH65B antibody, pale/ple antibody, th antibody, dyt14 antibody, dyt5b antibody, tyh antibody, tyrosine hydroxylase antibody, pale antibody, tyrosine 3-monooxygenase antibody, tyrosine hydroxylase S homeolog antibody, TH antibody, Th antibody, ple antibody, th antibody, th.S antibody, LOC100008895 antibody

Background: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001, Dunkley et al., 2004).Synonyms: TYH, Tyrosine 3-hydroxylase

Gene ID: 25085

NCBI Accession: NP_036872

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour