CD97 antibody (Extracellular Domain, N-Term)

Catalog No. ABIN401030

Product Details anti-CD97 Antibody

Target: CD97

Binding Specificity: AA 1-512, Extracellular Domain, N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD97 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against the CD97 protein. A BLAST analysis was used to suggest cross reactivity with CD97 proteins from Mouse (100 %) and Rat (74 %). Only 49 % homology is noted for the Human homologue. Reactivity against CD97 from other sources is not known.

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse, Human, Chimpanzee.

Purification: Affinity chromatography on Protein A

Immunogen: Recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein

Isotype: IgG

Application Details

Application Notes: Immunofluorescence: 1/1000 - 1/5000. Western Blot: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL deionized water (or equivalent).

Concentration: 5.0 mg/mL (UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M NaCl, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CD97

Target: CD97

Alternative Name: CD97 (CD97 Products)

Synonyms: AA409984 antibody, TM7LN1 antibody, adhesion G protein-coupled receptor E5 antibody, Adgre5 antibody, ADGRE5 antibody

Background: CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including hematopoietic cells. Particularly high levels of expression are also seen in the lung and the thymus gland. In the spleen, expression is detected on most myeloid cells and variable portions of T cells, B cells and NK cells. In humans, CD97 expression is upregulated during lymphocyte activation on various epithelial cancer cells including colon, thyroid, stomach, and esophagus. One high affinity ligand that has been identified for CD97 is the integrin alpha 5 beta 1. CD97 engagement of endothelial alpha 5 beta 1 results in increased angiogenesis. Signals generated through CD97 are thought to result in Rho activation and the regulation of motility. Antibodies against CD97 are suitable for exploring various mouse genetic models of cancer, angiogenesis, and inflammation. CD97 can be proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. Multiple isoforms have been identified for this protein.Synonyms: Leukocyte antigen CD97

Gene ID: 26364

NCBI Accession: NP_001156501

UniProt: Q9Z0M6