GGA3 antibody (AA 400-415)

Catalog No. ABIN401317

Product Details anti-GGA3 Antibody

Target: GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))

Binding Specificity: AA 400-415

Reactivity: Human, Orang-Utan, Macaque, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GGA3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against GGA3 protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Macaque, Orangutan.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 400-415 of Human GGA3

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10000 - 1/50000. Western Blot: 1/500 - 1/3000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.62 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for GGA3

Target: GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))

Alternative Name: GGA3 (GGA3 Products)

Synonyms: C230037M19Rik antibody, mKIAA0154 antibody, golgi associated, gamma adaptin ear containing, ARF binding protein 3 antibody, Gga3 antibody, GGA3 antibody

Background: GGA3 (also known as ADPribosylation factor binding protein GGA3, Golgilocalized, gamma ear-containing, ARF-binding protein 3, Gamma-adaptin related protein 3) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARFdependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200, GGA1 and GGA2 as well as RABEP1, RABGEF1 and type-I membrane proteins M6PR/CD-MPR, IGF2R/CIMPR and BACE1. GGA3 is a ubiquitously expressed membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.Synonyms: ADP-ribosylation factor-binding protein GGA3, Golgi-localized gamma ear-containing ARF-binding protein 3, KIAA0154

Gene ID: 23163

NCBI Accession: NP_001166174

UniProt: Q9NZ52