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ASAP1 antibody (AA 777-787)

ASAP1 Reactivity: Mouse, Human, Cow, Rat, Dog WB, IF, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN401327
  • Target See all ASAP1 Antibodies
    ASAP1 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1 (ASAP1))
    Binding Specificity
    • 15
    • 8
    • 7
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 777-787
    Reactivity
    • 43
    • 35
    • 10
    • 6
    • 1
    • 1
    • 1
    Mouse, Human, Cow, Rat, Dog
    Host
    • 52
    • 2
    • 1
    Rabbit
    Clonality
    • 53
    • 2
    Polyclonal
    Conjugate
    • 20
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This ASAP1 antibody is un-conjugated
    Application
    • 43
    • 26
    • 13
    • 13
    • 3
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
    Cross-Reactivity (Details)
    Species reactivity (expected):Human, Chicken, Bovine, Canine, Rat, Chimpanzee.
    Species reactivity (tested):Mouse.
    Purification
    Affinity chromatography
    Immunogen
    Synthetic peptide corresponding to amino acids 777-787 of mouse ASAP1 protein
    Isotype
    IgG
    Top Product
    Discover our top product ASAP1 Primary Antibody
  • Application Notes
    ELISA: 1/2,000 - 1/10,000. Western Blot: 1/500 - 1/2,000. Immunofluorescence.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.95 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
  • Target
    ASAP1 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1 (ASAP1))
    Alternative Name
    ASAP1 / DDEF1 (ASAP1 Products)
    Synonyms
    AV239055 antibody, DEF-1 antibody, Ddef1 antibody, PAP antibody, mKIAA1249 antibody, s19 antibody, AMAP1 antibody, CENTB4 antibody, DDEF1 antibody, PAG2 antibody, ZG14P antibody, ASAP1 antibody, ddef1 antibody, Asap1 antibody, si:ch211-160i2.3 antibody, ArfGAP with SH3 domain, ankyrin repeat and PH domain1 antibody, ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 antibody, toxic extracellular endopeptidase antibody, ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 L homeolog antibody, ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a antibody, Asap1 antibody, ASAP1 antibody, asaP1 antibody, asap1 antibody, asap1.L antibody, asap1a antibody
    Background
    ASAP1 (also known as AMAP1, 130- kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP,ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, DEF-1) is an Arf-directed GTPase activating protein that is a substrate for the kinases Src and FAK and has been implicated in the regulation of membrane traffic, focal adhesions and invadopodia/podosomes. Phosphorylation of ASAP1 at tyrosine 782 has been found to affect enzymatic and some biological activities, including the function of invadopodia. ASAP1 is expressed in many tissues but is most abundant in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals. Multiple transcript variants have been reported for this protein.Synonyms: 130 kDa phosphatidylinositol 4, 5-biphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1, AMAP1, ANK repeat and PH domain-containing protein 1, Arf-GAP with SH3 domain, CENTB4, Development and differentiation-enhancing factor 1, KIAA1249, PAG2, PIP2-dependent ARF1 GAP, ZG14P
    Gene ID
    10090
    UniProt
    Q9QWY8
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