IER5 antibody (Internal Region)

Catalog No. ABIN452683

Product Details anti-IER5 Antibody

Target: IER5 (Immediate Early Response 5 (IER5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IER5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes IER5.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QPPSGGEDDDAEE, from the internal region of the protein sequence according to NP_057629.1.

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/2000. Western Blot: 1-3 μg/mL. Customer's experiments gave a band at approx 45 kDa in lysatesof transfected H1299 overexpressing IER5 but not when transfected with empty vector(calculated MW of 33.5 kDa according to NP_057629.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for IER5

Target: IER5 (Immediate Early Response 5 (IER5))

Alternative Name: IER5 (IER5 Products)

Synonyms: si:dz129i22.1 antibody, wu:fa99a02 antibody, wu:fb04b03 antibody, wu:fb11f08 antibody, zgc:136422 antibody, sbbi48 antibody, MGC83180 antibody, IER5 antibody, SBBI48 antibody, immediate early response 5 antibody, immediate early response 5 L homeolog antibody, ier5 antibody, ier5.L antibody, IER5 antibody, Ier5 antibody

Background: IER5 is a similar protein to other immediate early response proteins. In mouse, a similar protein may play an important role in mediating the cellular response to mitogenic signals.Synonyms: Immediate early response gene 5 protein, PP4583, SBBI48

Gene ID: 51278

NCBI Accession: NP_057629

UniProt: Q5VY09