STAT3 antibody (C-Term, Isoform 1)

Catalog No. ABIN374087

Product Details anti-STAT3 Antibody

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Binding Specificity: C-Term, Isoform 1

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This STAT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: DMELTSECAT SPM

Specificity: This antibody is expected to recognize Isoform 1 (NP_644805.1) and Isoform 2 (NP_003141.2).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Pig and Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Immunoaffinity Chromatography

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_003141.2, NP_644805.1. Genename: STAT3

Application Details

Application Notes: Peptide ELISA: 1/128000. Western blot: 1-3 μg/mL. Approx.90 kDa band observed in lysates of NIH3T3 (calculatedMW of 88.0 kDa according to NP_644805.1 and of 88.1 according to NP_003141.2). Immunohistochemistry: Rommended overnight staining at 10 μg/mL (See picture). Stainsmouse thymic medulla and not the cortex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: STAT3 (STAT3 Products)

Synonyms: 1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody

Background: The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL10, IL6, IL11, IL12, IL2, EGF, among others. STAT3 beta appears to be activated by both EGF and IL6, but STAT3 alpha is only activated by EGF. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE acute phase response element) and SIE (csis inducible element). STAT4, predominantly expressed in testis and myeloid cells, is activated by ILl2. STAT5 is activated by prolactin and IL3. STAT6 participates in signaling pathways mediated by IL4.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3

Gene ID: 6774

NCBI Accession: NP_003141

UniProt: P40763

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor