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GRB2 antibody (C-Term)

GRB2 Reactivity: Human, Mouse, Rat, Cow, Dog WB, IHC (p), EIA Host: Goat Polyclonal unconjugated
Catalog No. ABIN374150
  • Target See all GRB2 Antibodies
    GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))
    Binding Specificity
    • 8
    • 6
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 64
    • 31
    • 30
    • 7
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Dog
    Host
    • 52
    • 9
    • 3
    Goat
    Clonality
    • 53
    • 10
    • 1
    Polyclonal
    Conjugate
    • 45
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GRB2 antibody is un-conjugated
    Application
    • 49
    • 33
    • 18
    • 12
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Sequence
    C-PRNYVTPVNR NV
    Specificity
    This antibody detects GRB2 / ASH at C-term.
    Cross-Reactivity (Details)
    Species reactivity (expected):Rat, Canine (Dog), Cow.
    Species reactivity (tested):Human, Mouse
    Purification
    Immunoaffinity Chromatography
    Immunogen
    Synthetic peptide from C-Terminus of the protein sequence according to NP_002077.1, NP_987102.1 Genename: GRB2
    Top Product
    Discover our top product GRB2 Primary Antibody
  • Application Notes
    Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 30 kDa band seen in Jurkat lysate. Immunohistochemistry: 0.1-0.3 μg/mL. In paraffin embedded Human Tonsil, stains mantlezone and to a lessser extent germinal centre.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))
    Alternative Name
    GRB2 / ASH (GRB2 Products)
    Synonyms
    GRB2 antibody, grb2 antibody, AA408164 antibody, Ash antibody, ASH antibody, EGFRBP-GRB2 antibody, Grb3-3 antibody, MST084 antibody, MSTP084 antibody, NCKAP2 antibody, wu:fb18e12 antibody, wu:fb34e06 antibody, zgc:73077 antibody, growth factor receptor bound protein 2 antibody, growth factor receptor-bound protein 2 antibody, growth factor receptor-bound protein 2b antibody, GRB2 antibody, grb2 antibody, Grb2 antibody, grb2b antibody
    Background
    Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.Synonyms: Growth factor receptor-bound protein 2
    Gene ID
    2885
    NCBI Accession
    NP_002077
    UniProt
    P62993
    Pathways
    RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation
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