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FBXO43 antibody (C-Term)

FBXO43 Reactivity: Human, Mouse, Rat, Cow, Dog, Pig WB, IF, FACS, EIA Host: Goat Polyclonal unconjugated
Catalog No. ABIN374351
  • Target See all FBXO43 Antibodies
    FBXO43 (F-Box Protein 43 (FBXO43))
    Binding Specificity
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    Human, Mouse, Rat, Cow, Dog, Pig
    Host
    • 10
    • 2
    • 1
    Goat
    Clonality
    • 11
    • 2
    Polyclonal
    Conjugate
    • 13
    This FBXO43 antibody is un-conjugated
    Application
    • 11
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
    Sequence
    C-PGSAQSKRNL KRL
    Specificity
    Recognizes EMI2.
    Cross-Reactivity (Details)
    Species reactivity (expected):Human, Mouse, Canine, Pig, Cow.
    Species reactivity (tested):Rat.
    Purification
    Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
    Immunogen
    Peptide C Terminus of the protein sequence according to NP_001025031.2. Genename: FBXO43
    Top Product
    Discover our top product FBXO43 Primary Antibody
  • Application Notes
    Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Has been successfully used on Rat Pachytene Spermatocytesshowing a 71 kDa band (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389). This product has been successfully used in Immunofluorescence and Flow Cytometry onRat meiotic Testis cells (5-10 μg/mL): (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    FBXO43 (F-Box Protein 43 (FBXO43))
    Alternative Name
    FBXO43 (FBXO43 Products)
    Synonyms
    EMI2 antibody, ERP1 antibody, FBX43 antibody, 4930533G20Rik antibody, Emi2 antibody, emi2 antibody, erp1 antibody, Xerp1 antibody, zgc:66488 antibody, F-box protein 43 antibody, F-box protein 43 L homeolog antibody, FBXO43 antibody, Fbxo43 antibody, fbxo43 antibody, fbxo43.L antibody
    Background
    Members of the F-box protein family, such as Fbp5B, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.Synonyms: EMI2, Endogenous meiotic inhibitor 2, F-box only protein 43, FBP5B
    Gene ID
    286151
    NCBI Accession
    NP_001025031
    UniProt
    Q4G163
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