Anillin antibody (C-Term)

Catalog No. ABIN374360

Product Details anti-Anillin Antibody

Target: Anillin (ANLN) (Anillin, Actin Binding Protein (ANLN))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Anillin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: WQPDACYKPI GKP

Specificity: This antibody detects Anillin / ANLN at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_061155.2. Genename: ANLN

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 64000. Western blot: Experiments gave an approx 75 kDa band in Mouse Brain lysates after 0.3 μg/mL antibody staining. It was successfully blocked by incubation with the immunizingpeptide. Immunohistochemistry: 3-10 μg/mL. In paraffin embedded Human Kidney shows staining ofnuclei in some cells of renal tubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for Anillin

Target: Anillin (ANLN) (Anillin, Actin Binding Protein (ANLN))

Alternative Name: Anillin / ANLN (ANLN Products)

Synonyms: Scraps antibody, scra antibody, ANLN antibody, 1110037A17Rik antibody, 2900037I21Rik antibody, C78101 antibody, scraps antibody, ABP8 antibody, Abp8 antibody, Ani antibody, Anil antibody, Anillin antibody, CG2092 antibody, Dmel\\CG2092 antibody, Scra antibody, ani antibody, anil antibody, anillin antibody, l(2)03427 antibody, l(2)43Ec antibody, l(2)k08255 antibody, RGD1561367 antibody, si:ch211-197n10.2 antibody, wu:fb74d05 antibody, wu:fd10d09 antibody, wu:fi41f04 antibody, anillin actin binding protein antibody, anillin, actin binding protein antibody, scraps antibody, anillin actin binding protein L homeolog antibody, ANLN antibody, Anln antibody, anln antibody, scra antibody, anln.L antibody

Background: Anillin is required for cytokinesis. It is essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.Synonyms: Actin-binding protein anillin

Gene ID: 54443

NCBI Accession: NP_061155

UniProt: Q9NQW6