ATP6AP2 antibody (C-Term)

Catalog No. ABIN374363

Product Details anti-ATP6AP2 Antibody

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP6AP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-SIIYRMTNQK IRMD

Specificity: This antibody detects Renin Receptor at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Cow.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_005756.2 Genename: ATP6AP2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.25-1 μg/mL. Approx 37 kDa band observed in Human Kidney lysates(predicted size of approx.40Kda according to NP_005756).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-ATP6AP2 antibody (ABIN374363)

Happold, Roth, Wick, Schmidt, Florea, Silginer, Reifenberger, Weller: "Distinct molecular mechanisms of acquired resistance to temozolomide in glioblastoma cells." in: Journal of neurochemistry, Vol. 122, Issue 2, pp. 444-55, (2013) (PubMed).

Zhu, Yoneda, Demura, Karashima, Usukura, Yamagishi, Takeda: "Effect of mineralocorticoid receptor blockade on the renal renin-angiotensin system in Dahl salt-sensitive hypertensive rats." in: Journal of hypertension, Vol. 27, Issue 4, pp. 800-5, (2009) (PubMed).

Target Details for ATP6AP2

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Alternative Name: Renin Receptor (ATP6AP2 Products)

Synonyms: APT6M8-9 antibody, ATP6IP2 antibody, ATP6M8-9 antibody, ELDF10 antibody, M8-9 antibody, MRXE antibody, MSTP009 antibody, XMRE antibody, (P)RR antibody, 5730403E06Rik antibody, Atp6ip2 antibody, tj6 antibody, wss antibody, arcl antibody, j6b7 antibody, stv1 antibody, tj6m antibody, tj6s antibody, vph1 antibody, atp6a2 antibody, atp6n1d antibody, ATP6AP2 antibody, DKFZp459G0918 antibody, atp6ap2 antibody, wu:fj36c03 antibody, wu:fq15e04 antibody, zgc:73194 antibody, ATPase H+ transporting accessory protein 2 antibody, ATPase, H+ transporting, lysosomal accessory protein 2 antibody, ATPase H+ transporting V0 subunit d2 antibody, ATPase H+ transporting V0 subunit a2 antibody, ATPase, H+ transporting, lysosomal accessory protein 2 S homeolog antibody, ATP6AP2 antibody, Atp6ap2 antibody, ATP6V0D2 antibody, atp6v0a2 antibody, atp6ap2.S antibody, atp6ap2 antibody

Background: Synonyms: ATP6AP2, ATP6IP2, ATP6M8-9, ATPase H(+)-transporting lysosomal accessory protein 2, ATPase H(+)-transporting lysosomal-interacting protein 2, CAPER, ELDF10, ER-localized type I transmembrane adaptor, Embryonic liver differentiation factor 10, HT028, MSTP009, N14F, PSEC0072, Renin/prorenin receptor, Vacuolar ATP synthase membrane sector-associated protein M8-9

Gene ID: 10159

NCBI Accession: NP_005756

UniProt: O75787

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones