Centaurin beta 2 antibody (C-Term)

Catalog No. ABIN374367

Product Details anti-Centaurin beta 2 Antibody

Target: Centaurin beta 2 (ACAP2) (ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 2 (ACAP2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Centaurin beta 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-EKLNRFQQDS QKF

Specificity: This antibody detects ACAP2 / CENTB2 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, DogSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_036419.3 Genename: ACAP2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 90 kDa band observed in Human T cell line MOLT4 lysates(calculated MW of 88.0 kDa according to NP_036419). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Lung shows strongstaining of Type II pneumocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for Centaurin beta 2

Target: Centaurin beta 2 (ACAP2) (ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 2 (ACAP2))

Alternative Name: ACAP2 / CENTB2 (ACAP2 Products)

Synonyms: CENTB2 antibody, CNT-B2 antibody, 4832442G16 antibody, 9530039J15Rik antibody, Centb2 antibody, mKIAA0041 antibody, centb2 antibody, MGC81056 antibody, ACAP2 antibody, MGC139548 antibody, cenB1A antibody, si:dkey-254a11.3 antibody, ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 antibody, ArfGAP with coiled-coil, ankyrin repeat and PH domains 3 L homeolog antibody, ACAP2 antibody, Acap2 antibody, acap3.L antibody, acap2 antibody

Background: Centaurin beta 2 is a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins. It contains two N-terminal coiled-coil regions, a pleckstrin homology (PH) domain, an ARF-GAP domain and C-terminal ankyrin repeats.Synonyms: ANK repeat and PH domain-containing protein 2, ARFGAP with coiled-coil, Centaurin beta 2, KIAA0041

Gene ID: 23527

NCBI Accession: NP_036419

UniProt: Q15057