ADH5 antibody (Internal Region)

Catalog No. ABIN374869

Product Details anti-ADH5 Antibody

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ADH5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-KKIKVDEFVT HN

Specificity: This antibody detects Alcohol Dehydrogenase 5 (ADH5).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_000662.3 Genename: ADH5

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 1000. Western blot: 1-3 μg/mL. Customer's experiments gave a band at approx 38 kDa in MouseLymphocyte lysates (calculated MW of 39.8 kDa according to Human NP_000662.3 and39.8 kDa according to Mouse NP_031436.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ADH5

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Alternative Name: Alcohol Dehydrogenase 5 (ADH5) (ADH5 Products)

Synonyms: ADH2 antibody, ADH-3 antibody, ADHX antibody, FALDH antibody, FDH antibody, GSH-FDH antibody, GSNOR antibody, ADH1C antibody, ADH3 antibody, wu:fb60b11 antibody, Adh-5 antibody, Adh3 antibody, adh-3 antibody, adh3 antibody, adh5 antibody, adhx antibody, fdh antibody, gsnor antibody, ADH4 antibody, ADH5 antibody, alcohol dehydrogenase 1B (class I), beta polypeptide antibody, alcohol dehydrogenase 5 (class III), chi polypeptide antibody, alcohol dehydrogenase 5 antibody, alcohol dehydrogenase 5 (class III), chi polypeptide L homeolog antibody, alcohol dehydrogenase class-3 antibody, ADH1B antibody, ADH5 antibody, adh5 antibody, Adh5 antibody, adh5.L antibody, LOC101112223 antibody

Background: The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.Synonyms: Alcohol dehydrogenase class 3 chi chain, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase

Molecular Weight: 39.7 kDa according to NP_000662.3.

Gene ID: 128, 9606

UniProt: P11766