Arylsulfatase B antibody (Internal Region)

Catalog No. ABIN374887

Product Details anti-Arylsulfatase B Antibody

Target: Arylsulfatase B (ARSB)

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Arylsulfatase B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-KLARGHTNGT KPLD

Specificity: This antibody detects internal Arylsulfatase B. It is expected to recognise both reported isoforms (NP_000037.2, NP_942002.1).

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_000037.2, NP_942002.1.

Application Details

Application Notes: Peptide ELISA: limit dilution 1: 64000. Western blot: 0.03-0.1 μg/mL. Approx 45 kDa band observed in Human Heart lysates(calculated MW of 46.5 kDa according to NP_942002.1). An additional band of unknownidentity was also consistently observed at 35 kDa. This band was successfully blocked byincubation with the immunising peptide. We would appreciate any feedback from peoplein the field - have any such results been reported with other antibodies/lysates? Have anyfurther splice variants/modified forms been reported?Immunohistochemistryon paraffin sections: 5-10 μg/mL. In Human Skeletal Muscle showsstriated pattern staining of majority of muscular fibres.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-Arylsulfatase B antibody (ABIN374887)

Zhao, Wang, Li, Dong, Wu: "Arylsulfatase B Mediates the Sulfonation-Transport Interplay in Human Embryonic Kidney 293 Cells Overexpressing Sulfotransferase 1A3." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 44, Issue 9, pp. 1441-9, (2018) (PubMed).

Target Details for Arylsulfatase B

Target: Arylsulfatase B (ARSB)

Alternative Name: Arylsulfatase B (ARSB Products)

Synonyms: ASB antibody, G4S antibody, MPS6 antibody, 1110007C02Rik antibody, AI480648 antibody, As-1 antibody, As-1r antibody, As-1s antibody, As-1t antibody, As1 antibody, As1-r antibody, As1-s antibody, As1-t antibody, Asr-1 antibody, Ast-1 antibody, ARSB antibody, arylsulfatase B antibody, arylsulfatase antibody, arylsulfatase b antibody, ARSB antibody, Arsb antibody, RB348 antibody, LOC5566067 antibody, CpipJ_CPIJ011047 antibody, VDBG_03275 antibody, arsb antibody, LOC5579667 antibody

Background: Arylsulfatase B (ARSB) belongs to the sulfatase family. The arylsulfatase B homodimer catalyzes hydrolysis of the sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. Defects in this gene cause Maroteaux-Lamy syndrome.Synonyms: ARSB, ASB, G4S, N-acetylgalactosamine-4-sulfatase

Gene ID: 411, 9606

UniProt: P15848

Pathways: Glycosaminoglycan Metabolic Process