SFRP4 antibody (Internal Region)

Catalog No. ABIN375089

Product Details anti-SFRP4 Antibody

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This SFRP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes SFRP4.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Bovine and Porcine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence TNSSCQCPHILPHQD, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: Detection limit dilution: 1/16000. Western blot: 0.3-1 μg/mL. Approx.40 kDa band observed in Human Uterus lysates(calculated MW of 39.0 kDa according to NP_003005.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 with 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SFRP4

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Alternative Name: SFRP4 (SFRP4 Products)

Synonyms: SFRP4 antibody, FRP-4 antibody, FRPHE antibody, sFRP-4 antibody, secreted frizzled related protein 4 antibody, secreted frizzled-related protein 4 antibody, SFRP4 antibody, Sfrp4 antibody

Background: Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.Synonyms: FRP4, FRPHE, Frizzled protein human endometrium, SFRP-4, Secreted frizzled-related protein 4

Gene ID: 6424, 9606

UniProt: Q6FHJ7

Pathways: WNT Signaling