APOB antibody (Internal Region)

Catalog No. ABIN375116

Product Details anti-APOB Antibody

Target: APOB (Apolipoprotein B (APOB))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOB antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-TDLHLRYQKD KK

Specificity: This antibody recognizes Human Apolipoprotein B (Apo B).

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000375.2. Genename: APOB

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.3 - 1 μg/mL, Bands corresponding to the exopected sizes of ApoB100 andApoB48 were detected in Human serum samples from five different individuals.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: Apolipoprotein B (Apo B) (APOB Products)

Synonyms: FLDB antibody, LDLCQ4 antibody, Ac1-060 antibody, Apo B-100 antibody, ApoB-100 antibody, ApoB-48 antibody, AI315052 antibody, apob-100 antibody, apob-48 antibody, APOB-100 antibody, ApoB(100) antibody, APOB antibody, apolipoprotein B antibody, APOB antibody, Apob antibody

Background: Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 516 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/L.Synonyms: Apo-B, ApoB, ApoB-100, ApoB100

Gene ID: 338, 9606

UniProt: P04114

Pathways: Lipid Metabolism