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MAPKAP Kinase 2 antibody (AA 310-325)

MAPKAPK2 Reactivity: Human, Mouse, Rat, Rabbit, Zebrafish (Danio rerio), Chicken, Hamster, Xenopus laevis WB, IP, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN117983
  • Target See all MAPKAP Kinase 2 (MAPKAPK2) Antibodies
    MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))
    Binding Specificity
    • 35
    • 25
    • 19
    • 16
    • 7
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 310-325
    Reactivity
    • 106
    • 95
    • 91
    • 21
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Rabbit, Zebrafish (Danio rerio), Chicken, Hamster, Xenopus laevis
    Host
    • 134
    • 3
    • 2
    Rabbit
    Clonality
    • 120
    • 19
    Polyclonal
    Conjugate
    • 60
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    This MAPKAP Kinase 2 antibody is un-conjugated
    Application
    • 90
    • 43
    • 40
    • 39
    • 33
    • 24
    • 19
    • 13
    • 9
    • 8
    • 8
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
    Sequence
    M-T-S-A-L-A-T-M-R-V-D-Y-E-Q-I-K
    Purification
    Affinity Purified
    Immunogen
    This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to aa 310-325 of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
    Top Product
    Discover our top product MAPKAPK2 Primary Antibody
  • Application Notes
    Suitable for use in Western blotting, ELISA, Immunoprecipitation and kinase assays. Although not tested, this antibody is likely functional in Immunohistochemistry and otherimmunological methods. Affinity purified anti-MAPKAP Kinase 2 is specific for MAPKAPKinase 2 and will not effectively immunoblot other isoforms of the kinase (i. e. MAPKAP K1or K3) from cell lines and/or tissues. Use a HeLa cell lysate as a positive control. MK2assays are often used and are easier and have less background than p38 kinase assays. Immunoprecipitation of MK2 using antibodies is used for this purpose. This product wasassayed by immunoblot and was found to be reactive to 20 g of crude protein extractprepared from HeLa cells. The gel was probed with Affinity Purified anti-MAPKAP Kinase2used at a dilution of 1: 2000. Use Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG(H&L) (GOAT) (Min X Bv Ch Gt GP Hs Hu Ms Rt & Sh Serum Proteins) as a secondary
    Restrictions
    For Research Use only
  • Concentration
    0.9 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 2-8 °C. DO NOT FREEZE! This products is photosensitive and should be protected from light.
  • Concejero, Chen, Wang, Wang, Lin, Liu, Yang, Yong, Lin, Jawan, Huang, Cheng, Eng: "Living donor liver transplantation for hepatocellular carcinoma: a single-center experience in Taiwan." in: Transplantation, Vol. 85, Issue 3, pp. 398-406, (2008) (PubMed).

  • Target
    MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))
    Alternative Name
    MAPKAP Kinase-2 (MAPKAPK2 Products)
    Synonyms
    mapkapk2 antibody, wu:fb30e10 antibody, wu:fc56c10 antibody, wu:fi03a01 antibody, MGC78852 antibody, MAPKAPK2 antibody, AA960234 antibody, MAPKAP-K2 antibody, MK-2 antibody, MK2 antibody, Rps6kc1 antibody, mitogen-activated protein kinase-activated protein kinase 2a antibody, mitogen-activated protein kinase-activated protein kinase 2 L homeolog antibody, mitogen-activated protein kinase-activated protein kinase 2 antibody, MAP kinase-activated protein kinase 2 antibody, mapkapk2a antibody, mapkapk2.L antibody, MAPKAPK2 antibody, Mapkapk2 antibody
    Background
    MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The bestcharacterized MK2 substrate is hsp27. Although unknown, the physiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNF-α at a posttranscriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.Synonyms: MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MAPKAPK2, MK2
    Gene ID
    9986
    UniProt
    P49139
    Pathways
    MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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