Caspase 9 antibody

Catalog No. ABIN5573805

Product Details anti-Caspase 9 Antibody

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)

Purpose: Rabbit polyclonal antibody raised against recombinant CASP9.

Specificity: Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active Caspase-9 staining may also be seen in the mitochondria. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation.

Cross-Reactivity: Dog, Gerbil, Human, Mouse, Rat

Immunogen: Recombinant protein corresponding to human CASP9.

Application Details

Application Notes: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:5000)
Immunoprecipitation (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In serum (0.05 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: CASP9 (CASP9 Products)

Synonyms: CASP9 antibody, AI115399 antibody, APAF-3 antibody, AW493809 antibody, CASP-9 antibody, Caspase-9 antibody, ICE-LAP6 antibody, Mch6 antibody, Apaf3 antibody, Casp-9-CTD antibody, Casp9_v1 antibody, Ice-Lap6 antibody, APAF3 antibody, MCH6 antibody, PPP1R56 antibody, caspase 9 antibody, CASP9 antibody, Casp9 antibody

Background: Full Gene Name: caspase 9, apoptosis-related cysteine peptidase
Synonyms: APAF-3,APAF3,CASPASE-9c,ICE-LAP6,MCH6

Gene ID: 842

NCBI Accession: NP_001220

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity