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CD73 antibody (AA 30-250)

Name: CD73 antibody (AA 30-250)
SKU: ABIN5584792
Price: 812 USD
Availability: InStock

NT5E Reactivity: Human WB, FACS, ELISA, IHC (p) Host: Mouse Monoclonal 4G6E3 unconjugated

Western blot analysis of human NT5E (AA: 30-250) recombinant protein (Expected MW is 26.

Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [NT5E (AA: 30-250)-hIgGFc transfected HEK293 cells] with NT5E monoclonal antibody, clone 4G6E3 at 1:500-1:2000 dilution.

Flow Cytometry analysis of A431 cells with NT5E monoclonal antibody, clone 4G6E3 at 1:200-1:400 dilution (Green) and negative control (Red).

FACS

Immunohistochemical staining of human endometrial cancer tissues (A) and esophageal cencer tissues (B) with NT5E monoclonal antibody, clone 4G6E3 at 1:200-1:1000 dilution.

IHC

ELISA

5 images

Catalog No. ABIN5584792

Datasheet as PDF

Target See all CD73 (NT5E) Antibodies

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
Binding Specificity
16

14

14

11

10

6

5

4

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 30-250
Reactivity
112

51

26

18

18

16

4

2

1

1

1

1

1
Human
Host
92

31

9

2
Mouse
Compatible Secondaries
Clonality
83

51
Monoclonal
Conjugate
59

16

13

9

9

6

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This CD73 antibody is un-conjugated
Application
94

58

52

50

18

16

14

14

14

7

5

3

3

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose

Mouse monoclonal antibody raised against partial recombinant human NT5E.

Cross-Reactivity

Human

Immunogen

Recombinant protein corresponding to amino acids 30-250 of human TIE1.

Clone

4G6E3

Isotype

IgG1

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NT5E Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
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NT5E Reactivity: Human WB, FACS, IHC, IF, StM Host: Mouse Monoclonal NT5E-2503 unconjugated

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NT5E Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

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NT5E Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal AD2 PE

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibody
NT5E Reactivity: Cow WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E Reactivity: Human, Mouse, Rat IHC Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.

Restrictions

For Research Use only
Format

Liquid

Buffer

In PBS (0.05 % sodium azide)

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Target

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name

NT5E (NT5E Products)

Synonyms

CALJA antibody, CD73 antibody, E5NT antibody, NT antibody, NT5 antibody, NTE antibody, eN antibody, eNT antibody, zgc:63784 antibody, NT5E antibody, MGC114869 antibody, PSPTO2277 antibody, 2210401F01Rik antibody, AI447961 antibody, Nt5 antibody, 5'-nucleotidase ecto antibody, 5'-nucleotidase, ecto (CD73) antibody, 5'-nucleotidase antibody, 5' nucleotidase, ecto antibody, NT5E antibody, nt5e antibody, nt5e.S antibody, PSPTO_2277 antibody, CBU_1233 antibody, MCA1322 antibody, Nt5e antibody

Background

Full Gene Name: 5'-nucleotidase, ecto (CD73)
Synonyms: CD73,E5NT,NT,NT5,NTE,eN,eNT

Gene ID

4907

Pathways

Synaptic Membrane, Ribonucleoside Biosynthetic Process