Aldehyde Dehydrogenase antibody (HRP)

Catalog No. ABIN5596684

Product Details anti-Aldehyde Dehydrogenase Antibody (HRP)

Target: Aldehyde Dehydrogenase (ALDH)

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aldehyde Dehydrogenase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Aldehyde Dehydrogenase from other sources is unknown.

Purification: Aldehyde dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast].

Immunogen:

Immunogen: Aldehyde Dehydrogenase [Yeast]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Aldehyde dehydrogenase has been assayed against 1.0 μg of Aldehyde Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Aldehyde Dehydrogenase

Target: Aldehyde Dehydrogenase (ALDH)

Alternative Name: Aldehyde Dehydrogenase (ALDH Products)

Synonyms: 146084_at antibody, ACDH antibody, ALDH antibody, CG 3752 antibody, CG3752 antibody, Dmel\\CG3752 antibody, GB12741 antibody, Tb06.26G9.1140 antibody, Aldehyde dehydrogenase antibody, ALdehyde deHydrogenase antibody, aldehyde dehydrogenase, mitochondrial antibody, aldehyde dehydrogenase antibody, betaine-aldehyde dehydrogenase antibody, ran-specific GTPase-activating protein antibody, Aldh antibody, alh-6 antibody, alh-12 antibody, LOC550687 antibody, aldH antibody, Tb927.6.4210 antibody, HQ_RS05000 antibody, aldA-2 antibody, ALDH antibody, LOC618976 antibody

Background:

Synonyms: Aldehyde dehydrogenase [NAD(P)+] 1 ALD2, ALD5

Background: Aldehyde dehydrogenase are a group of enzymes that catalyse the oxidation (dehydrogenation) of aldehydes. Aldehyde dehydrogenase is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids, which leave the liver and are metabolized by the body's muscle and heart. ALDH2 also plays a crucial role in maintaining low blood levels of acetaldehyde during alcohol oxidation.

Gene Name: ALD2, ALD5

Gene ID: 855206

UniProt: P47771