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CRISPR-Cas9 (C-Term) antibody

Reactivity: Streptococcus pyogenes WB, ELISA, FM Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5596701
  • Target
    CRISPR-Cas9
    Binding Specificity
    • 6
    • 2
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 19
    • 2
    • 1
    • 1
    • 1
    • 1
    Streptococcus pyogenes
    Host
    • 15
    • 10
    Rabbit
    Clonality
    • 13
    • 12
    Polyclonal
    Conjugate
    • 17
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Un-conjugated
    Application
    • 23
    • 9
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
    Purification
    Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    Immunogen

    Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

    Immunogen Type: Peptide

    Isotype
    IgG
  • Application Notes

    Application Note: This affinity purified antibody has been tested for use in ELISA, Immunofluorescence, and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:1000

    ELISA Dilution: 1:25,000-1:30,000

    IF Microscopy Dilution: 1 μg/mL

    Comment

    User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    CRISPR-Cas9
    Alternative Name
    Cas 9
    Background

    Synonyms: CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, csn1, Cas-9 Antibody

    Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.

    Gene Name: cas9

    Gene ID
    901176
    UniProt
    Q99ZW2
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