Huntingtin antibody (Internal Region, pSer421)

Catalog No. ABIN5596736

Product Details anti-Huntingtin Antibody

Target: Huntingtin (HTT)

Binding Specificity: Internal Region, pSer421

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Huntingtin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with human based on 100% sequence homology. Cross-reactivity with Huntington pS421 from other sources has not been determined.

Purification: Anti-Huntington pS421 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of the S421 residue.

Immunogen:

Immunogen: Anti-Huntington pS421 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Huntingtin protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-HTT antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.07 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Huntingtin

Target: Huntingtin (HTT)

Alternative Name: Huntington (HTT Products)

Synonyms: HTT antibody, hd antibody, ZHD antibody, CG9995 antibody, Dmel\\CG9995 antibody, HD antibody, Hsap\\HD antibody, Htt antibody, dHtt antibody, dhtt antibody, SLC6A4 antibody, huntington antibody, it15 antibody, htt antibody, IT15 antibody, AI256365 antibody, C430023I11Rik antibody, Hd antibody, Hdh antibody, huntingtin antibody, HTT antibody, htt antibody, LOC373520 antibody, Htt antibody

Background:

Synonyms: Huntington disease protein, HTT, HD, IT15, HD protein, huntingtin, huntingtin (Huntington disease), IT15HD

Background: Huntington's disease (HD) is an autosomal dominant neurological disorder caused by a polyglutamine (polyQ) repeat expansion in the huntingtin (Htt) protein. The disease is characterized by neurodegeneration and formation of neuronal intracellular inclusions primarily in the striatum and cortex, leading to personality changes, motor impairment, and dementia. The Huntington's disease protein is ~350kD in size and is localized in the brain. Anti-Huntington antibody is ideal for investigators interested in Neuroscience research.

Gene Name: HTT

Pathways: PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport